rfp trap beads Search Results


rfp-trap magnetic beads  (Proteintech)
90
Proteintech rfp-trap magnetic beads
Rfp Trap Magnetic Beads, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rfp-trap magnetic beads/product/Proteintech
Average 90 stars, based on 1 article reviews
rfp-trap magnetic beads - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
gfp/rfp-trap agarose beads  (Proteintech)
90
Proteintech gfp/rfp-trap agarose beads
( A ) Conidia expressing Pth11-mCherry (pseudocolored yellow) were inoculated on an inductive surface and imaged at the indicated time-points. The inset in the 2 h panel highlights a tubulo-vesicular structure containing Pth11-mCherry. The asterisk depicts the association of Pth11 with the plasma membrane. The dotted line delineates the conidial boundary. Images are maximum intensity Z-projections of five confocal stacks, measuring 0.5 µm each. Scale bar, 10 µm. ( B ) Pth11 physically interacts with Rgs1. Immunoblots from a pull down experiment depicting the specific interaction between Pth11 and Rgs1 during appressorium initiation. Pth11 physically interacted with Rgs1 during pathogenic differentiation and failed to interact with Rgs1 during vegetative growth. In each case, equal concentrations of protein from both the strains were used for the pull down experiment. Total protein from an untagged wild-type strain served as a negative control in B. S1 and S2 represent two independent <t>Pth11-GFP</t> expressing strains. ( C ) An <t>RFP-trap</t> experiment depicting the specific interaction between Pth11-mCherry and Rgs1. After probing for Pth11-mCherry with α-mCherry antibody, the same membrane was stripped and re-probed using α-Rgs1 antibody. Total protein from a cytosolic-mCherry expressing strain served as a negative control in C. ( D ) Confocal microscopy based imaging of a BiFC experiment illustrating the in-vivo interaction between Pth11-nYFP and Rgs1-cYFP in the vegetative mycelium and at 4 h post inoculation on an inductive surface (right; DIC in left panel and YFP in right panel). Asterisk indicates YFP signal and thus likely interaction at the plasma membrane. Images are single plane images captured by a confocal microscope. Scale bar, 10 µm.
Gfp/Rfp Trap Agarose Beads, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gfp/rfp-trap agarose beads/product/Proteintech
Average 90 stars, based on 1 article reviews
gfp/rfp-trap agarose beads - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
rfp-trap1_a beads  (Proteintech)
90
Proteintech rfp-trap1_a beads
( A ) Conidia expressing Pth11-mCherry (pseudocolored yellow) were inoculated on an inductive surface and imaged at the indicated time-points. The inset in the 2 h panel highlights a tubulo-vesicular structure containing Pth11-mCherry. The asterisk depicts the association of Pth11 with the plasma membrane. The dotted line delineates the conidial boundary. Images are maximum intensity Z-projections of five confocal stacks, measuring 0.5 µm each. Scale bar, 10 µm. ( B ) Pth11 physically interacts with Rgs1. Immunoblots from a pull down experiment depicting the specific interaction between Pth11 and Rgs1 during appressorium initiation. Pth11 physically interacted with Rgs1 during pathogenic differentiation and failed to interact with Rgs1 during vegetative growth. In each case, equal concentrations of protein from both the strains were used for the pull down experiment. Total protein from an untagged wild-type strain served as a negative control in B. S1 and S2 represent two independent <t>Pth11-GFP</t> expressing strains. ( C ) An <t>RFP-trap</t> experiment depicting the specific interaction between Pth11-mCherry and Rgs1. After probing for Pth11-mCherry with α-mCherry antibody, the same membrane was stripped and re-probed using α-Rgs1 antibody. Total protein from a cytosolic-mCherry expressing strain served as a negative control in C. ( D ) Confocal microscopy based imaging of a BiFC experiment illustrating the in-vivo interaction between Pth11-nYFP and Rgs1-cYFP in the vegetative mycelium and at 4 h post inoculation on an inductive surface (right; DIC in left panel and YFP in right panel). Asterisk indicates YFP signal and thus likely interaction at the plasma membrane. Images are single plane images captured by a confocal microscope. Scale bar, 10 µm.
Rfp Trap1 A Beads, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rfp-trap1_a beads/product/Proteintech
Average 90 stars, based on 1 article reviews
rfp-trap1_a beads - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
rfp trap a beads  (Proteintech)
96
Proteintech rfp trap a beads
( A ) Conidia expressing Pth11-mCherry (pseudocolored yellow) were inoculated on an inductive surface and imaged at the indicated time-points. The inset in the 2 h panel highlights a tubulo-vesicular structure containing Pth11-mCherry. The asterisk depicts the association of Pth11 with the plasma membrane. The dotted line delineates the conidial boundary. Images are maximum intensity Z-projections of five confocal stacks, measuring 0.5 µm each. Scale bar, 10 µm. ( B ) Pth11 physically interacts with Rgs1. Immunoblots from a pull down experiment depicting the specific interaction between Pth11 and Rgs1 during appressorium initiation. Pth11 physically interacted with Rgs1 during pathogenic differentiation and failed to interact with Rgs1 during vegetative growth. In each case, equal concentrations of protein from both the strains were used for the pull down experiment. Total protein from an untagged wild-type strain served as a negative control in B. S1 and S2 represent two independent <t>Pth11-GFP</t> expressing strains. ( C ) An <t>RFP-trap</t> experiment depicting the specific interaction between Pth11-mCherry and Rgs1. After probing for Pth11-mCherry with α-mCherry antibody, the same membrane was stripped and re-probed using α-Rgs1 antibody. Total protein from a cytosolic-mCherry expressing strain served as a negative control in C. ( D ) Confocal microscopy based imaging of a BiFC experiment illustrating the in-vivo interaction between Pth11-nYFP and Rgs1-cYFP in the vegetative mycelium and at 4 h post inoculation on an inductive surface (right; DIC in left panel and YFP in right panel). Asterisk indicates YFP signal and thus likely interaction at the plasma membrane. Images are single plane images captured by a confocal microscope. Scale bar, 10 µm.
Rfp Trap A Beads, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rfp trap a beads/product/Proteintech
Average 96 stars, based on 1 article reviews
rfp trap a beads - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier
rfp trap magnetic agarose beads  (Proteintech)
95
Proteintech rfp trap magnetic agarose beads
( A ) Conidia expressing Pth11-mCherry (pseudocolored yellow) were inoculated on an inductive surface and imaged at the indicated time-points. The inset in the 2 h panel highlights a tubulo-vesicular structure containing Pth11-mCherry. The asterisk depicts the association of Pth11 with the plasma membrane. The dotted line delineates the conidial boundary. Images are maximum intensity Z-projections of five confocal stacks, measuring 0.5 µm each. Scale bar, 10 µm. ( B ) Pth11 physically interacts with Rgs1. Immunoblots from a pull down experiment depicting the specific interaction between Pth11 and Rgs1 during appressorium initiation. Pth11 physically interacted with Rgs1 during pathogenic differentiation and failed to interact with Rgs1 during vegetative growth. In each case, equal concentrations of protein from both the strains were used for the pull down experiment. Total protein from an untagged wild-type strain served as a negative control in B. S1 and S2 represent two independent <t>Pth11-GFP</t> expressing strains. ( C ) An <t>RFP-trap</t> experiment depicting the specific interaction between Pth11-mCherry and Rgs1. After probing for Pth11-mCherry with α-mCherry antibody, the same membrane was stripped and re-probed using α-Rgs1 antibody. Total protein from a cytosolic-mCherry expressing strain served as a negative control in C. ( D ) Confocal microscopy based imaging of a BiFC experiment illustrating the in-vivo interaction between Pth11-nYFP and Rgs1-cYFP in the vegetative mycelium and at 4 h post inoculation on an inductive surface (right; DIC in left panel and YFP in right panel). Asterisk indicates YFP signal and thus likely interaction at the plasma membrane. Images are single plane images captured by a confocal microscope. Scale bar, 10 µm.
Rfp Trap Magnetic Agarose Beads, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rfp trap magnetic agarose beads/product/Proteintech
Average 95 stars, based on 1 article reviews
rfp trap magnetic agarose beads - by Bioz Stars, 2026-03
95/100 stars
  Buy from Supplier
rfp trap beads  (Proteintech)
93
Proteintech rfp trap beads
( A ) Conidia expressing Pth11-mCherry (pseudocolored yellow) were inoculated on an inductive surface and imaged at the indicated time-points. The inset in the 2 h panel highlights a tubulo-vesicular structure containing Pth11-mCherry. The asterisk depicts the association of Pth11 with the plasma membrane. The dotted line delineates the conidial boundary. Images are maximum intensity Z-projections of five confocal stacks, measuring 0.5 µm each. Scale bar, 10 µm. ( B ) Pth11 physically interacts with Rgs1. Immunoblots from a pull down experiment depicting the specific interaction between Pth11 and Rgs1 during appressorium initiation. Pth11 physically interacted with Rgs1 during pathogenic differentiation and failed to interact with Rgs1 during vegetative growth. In each case, equal concentrations of protein from both the strains were used for the pull down experiment. Total protein from an untagged wild-type strain served as a negative control in B. S1 and S2 represent two independent <t>Pth11-GFP</t> expressing strains. ( C ) An <t>RFP-trap</t> experiment depicting the specific interaction between Pth11-mCherry and Rgs1. After probing for Pth11-mCherry with α-mCherry antibody, the same membrane was stripped and re-probed using α-Rgs1 antibody. Total protein from a cytosolic-mCherry expressing strain served as a negative control in C. ( D ) Confocal microscopy based imaging of a BiFC experiment illustrating the in-vivo interaction between Pth11-nYFP and Rgs1-cYFP in the vegetative mycelium and at 4 h post inoculation on an inductive surface (right; DIC in left panel and YFP in right panel). Asterisk indicates YFP signal and thus likely interaction at the plasma membrane. Images are single plane images captured by a confocal microscope. Scale bar, 10 µm.
Rfp Trap Beads, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rfp trap beads/product/Proteintech
Average 93 stars, based on 1 article reviews
rfp trap beads - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier
rfp trap magnetic beads  (Proteintech)
93
Proteintech rfp trap magnetic beads
( A ) Conidia expressing Pth11-mCherry (pseudocolored yellow) were inoculated on an inductive surface and imaged at the indicated time-points. The inset in the 2 h panel highlights a tubulo-vesicular structure containing Pth11-mCherry. The asterisk depicts the association of Pth11 with the plasma membrane. The dotted line delineates the conidial boundary. Images are maximum intensity Z-projections of five confocal stacks, measuring 0.5 µm each. Scale bar, 10 µm. ( B ) Pth11 physically interacts with Rgs1. Immunoblots from a pull down experiment depicting the specific interaction between Pth11 and Rgs1 during appressorium initiation. Pth11 physically interacted with Rgs1 during pathogenic differentiation and failed to interact with Rgs1 during vegetative growth. In each case, equal concentrations of protein from both the strains were used for the pull down experiment. Total protein from an untagged wild-type strain served as a negative control in B. S1 and S2 represent two independent <t>Pth11-GFP</t> expressing strains. ( C ) An <t>RFP-trap</t> experiment depicting the specific interaction between Pth11-mCherry and Rgs1. After probing for Pth11-mCherry with α-mCherry antibody, the same membrane was stripped and re-probed using α-Rgs1 antibody. Total protein from a cytosolic-mCherry expressing strain served as a negative control in C. ( D ) Confocal microscopy based imaging of a BiFC experiment illustrating the in-vivo interaction between Pth11-nYFP and Rgs1-cYFP in the vegetative mycelium and at 4 h post inoculation on an inductive surface (right; DIC in left panel and YFP in right panel). Asterisk indicates YFP signal and thus likely interaction at the plasma membrane. Images are single plane images captured by a confocal microscope. Scale bar, 10 µm.
Rfp Trap Magnetic Beads, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rfp trap magnetic beads/product/Proteintech
Average 93 stars, based on 1 article reviews
rfp trap magnetic beads - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier
rfp-trap beads  (Proteintech)
90
Proteintech rfp-trap beads
( A ) Conidia expressing Pth11-mCherry (pseudocolored yellow) were inoculated on an inductive surface and imaged at the indicated time-points. The inset in the 2 h panel highlights a tubulo-vesicular structure containing Pth11-mCherry. The asterisk depicts the association of Pth11 with the plasma membrane. The dotted line delineates the conidial boundary. Images are maximum intensity Z-projections of five confocal stacks, measuring 0.5 µm each. Scale bar, 10 µm. ( B ) Pth11 physically interacts with Rgs1. Immunoblots from a pull down experiment depicting the specific interaction between Pth11 and Rgs1 during appressorium initiation. Pth11 physically interacted with Rgs1 during pathogenic differentiation and failed to interact with Rgs1 during vegetative growth. In each case, equal concentrations of protein from both the strains were used for the pull down experiment. Total protein from an untagged wild-type strain served as a negative control in B. S1 and S2 represent two independent <t>Pth11-GFP</t> expressing strains. ( C ) An <t>RFP-trap</t> experiment depicting the specific interaction between Pth11-mCherry and Rgs1. After probing for Pth11-mCherry with α-mCherry antibody, the same membrane was stripped and re-probed using α-Rgs1 antibody. Total protein from a cytosolic-mCherry expressing strain served as a negative control in C. ( D ) Confocal microscopy based imaging of a BiFC experiment illustrating the in-vivo interaction between Pth11-nYFP and Rgs1-cYFP in the vegetative mycelium and at 4 h post inoculation on an inductive surface (right; DIC in left panel and YFP in right panel). Asterisk indicates YFP signal and thus likely interaction at the plasma membrane. Images are single plane images captured by a confocal microscope. Scale bar, 10 µm.
Rfp Trap Beads, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rfp-trap beads/product/Proteintech
Average 90 stars, based on 1 article reviews
rfp-trap beads - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
anti ofp magnetic beads  (Proteintech)
93
Proteintech anti ofp magnetic beads
( A ) Conidia expressing Pth11-mCherry (pseudocolored yellow) were inoculated on an inductive surface and imaged at the indicated time-points. The inset in the 2 h panel highlights a tubulo-vesicular structure containing Pth11-mCherry. The asterisk depicts the association of Pth11 with the plasma membrane. The dotted line delineates the conidial boundary. Images are maximum intensity Z-projections of five confocal stacks, measuring 0.5 µm each. Scale bar, 10 µm. ( B ) Pth11 physically interacts with Rgs1. Immunoblots from a pull down experiment depicting the specific interaction between Pth11 and Rgs1 during appressorium initiation. Pth11 physically interacted with Rgs1 during pathogenic differentiation and failed to interact with Rgs1 during vegetative growth. In each case, equal concentrations of protein from both the strains were used for the pull down experiment. Total protein from an untagged wild-type strain served as a negative control in B. S1 and S2 represent two independent <t>Pth11-GFP</t> expressing strains. ( C ) An <t>RFP-trap</t> experiment depicting the specific interaction between Pth11-mCherry and Rgs1. After probing for Pth11-mCherry with α-mCherry antibody, the same membrane was stripped and re-probed using α-Rgs1 antibody. Total protein from a cytosolic-mCherry expressing strain served as a negative control in C. ( D ) Confocal microscopy based imaging of a BiFC experiment illustrating the in-vivo interaction between Pth11-nYFP and Rgs1-cYFP in the vegetative mycelium and at 4 h post inoculation on an inductive surface (right; DIC in left panel and YFP in right panel). Asterisk indicates YFP signal and thus likely interaction at the plasma membrane. Images are single plane images captured by a confocal microscope. Scale bar, 10 µm.
Anti Ofp Magnetic Beads, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti ofp magnetic beads/product/Proteintech
Average 93 stars, based on 1 article reviews
anti ofp magnetic beads - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier
rfp trap bead slurry  (Proteintech)
96
Proteintech rfp trap bead slurry
( A ) Conidia expressing Pth11-mCherry (pseudocolored yellow) were inoculated on an inductive surface and imaged at the indicated time-points. The inset in the 2 h panel highlights a tubulo-vesicular structure containing Pth11-mCherry. The asterisk depicts the association of Pth11 with the plasma membrane. The dotted line delineates the conidial boundary. Images are maximum intensity Z-projections of five confocal stacks, measuring 0.5 µm each. Scale bar, 10 µm. ( B ) Pth11 physically interacts with Rgs1. Immunoblots from a pull down experiment depicting the specific interaction between Pth11 and Rgs1 during appressorium initiation. Pth11 physically interacted with Rgs1 during pathogenic differentiation and failed to interact with Rgs1 during vegetative growth. In each case, equal concentrations of protein from both the strains were used for the pull down experiment. Total protein from an untagged wild-type strain served as a negative control in B. S1 and S2 represent two independent <t>Pth11-GFP</t> expressing strains. ( C ) An <t>RFP-trap</t> experiment depicting the specific interaction between Pth11-mCherry and Rgs1. After probing for Pth11-mCherry with α-mCherry antibody, the same membrane was stripped and re-probed using α-Rgs1 antibody. Total protein from a cytosolic-mCherry expressing strain served as a negative control in C. ( D ) Confocal microscopy based imaging of a BiFC experiment illustrating the in-vivo interaction between Pth11-nYFP and Rgs1-cYFP in the vegetative mycelium and at 4 h post inoculation on an inductive surface (right; DIC in left panel and YFP in right panel). Asterisk indicates YFP signal and thus likely interaction at the plasma membrane. Images are single plane images captured by a confocal microscope. Scale bar, 10 µm.
Rfp Trap Bead Slurry, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rfp trap bead slurry/product/Proteintech
Average 96 stars, based on 1 article reviews
rfp trap bead slurry - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier
rfp trap agarose beads  (Proteintech)
96
Proteintech rfp trap agarose beads
( A ) Conidia expressing Pth11-mCherry (pseudocolored yellow) were inoculated on an inductive surface and imaged at the indicated time-points. The inset in the 2 h panel highlights a tubulo-vesicular structure containing Pth11-mCherry. The asterisk depicts the association of Pth11 with the plasma membrane. The dotted line delineates the conidial boundary. Images are maximum intensity Z-projections of five confocal stacks, measuring 0.5 µm each. Scale bar, 10 µm. ( B ) Pth11 physically interacts with Rgs1. Immunoblots from a pull down experiment depicting the specific interaction between Pth11 and Rgs1 during appressorium initiation. Pth11 physically interacted with Rgs1 during pathogenic differentiation and failed to interact with Rgs1 during vegetative growth. In each case, equal concentrations of protein from both the strains were used for the pull down experiment. Total protein from an untagged wild-type strain served as a negative control in B. S1 and S2 represent two independent <t>Pth11-GFP</t> expressing strains. ( C ) An <t>RFP-trap</t> experiment depicting the specific interaction between Pth11-mCherry and Rgs1. After probing for Pth11-mCherry with α-mCherry antibody, the same membrane was stripped and re-probed using α-Rgs1 antibody. Total protein from a cytosolic-mCherry expressing strain served as a negative control in C. ( D ) Confocal microscopy based imaging of a BiFC experiment illustrating the in-vivo interaction between Pth11-nYFP and Rgs1-cYFP in the vegetative mycelium and at 4 h post inoculation on an inductive surface (right; DIC in left panel and YFP in right panel). Asterisk indicates YFP signal and thus likely interaction at the plasma membrane. Images are single plane images captured by a confocal microscope. Scale bar, 10 µm.
Rfp Trap Agarose Beads, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rfp trap agarose beads/product/Proteintech
Average 96 stars, based on 1 article reviews
rfp trap agarose beads - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier

Image Search Results


( A ) Conidia expressing Pth11-mCherry (pseudocolored yellow) were inoculated on an inductive surface and imaged at the indicated time-points. The inset in the 2 h panel highlights a tubulo-vesicular structure containing Pth11-mCherry. The asterisk depicts the association of Pth11 with the plasma membrane. The dotted line delineates the conidial boundary. Images are maximum intensity Z-projections of five confocal stacks, measuring 0.5 µm each. Scale bar, 10 µm. ( B ) Pth11 physically interacts with Rgs1. Immunoblots from a pull down experiment depicting the specific interaction between Pth11 and Rgs1 during appressorium initiation. Pth11 physically interacted with Rgs1 during pathogenic differentiation and failed to interact with Rgs1 during vegetative growth. In each case, equal concentrations of protein from both the strains were used for the pull down experiment. Total protein from an untagged wild-type strain served as a negative control in B. S1 and S2 represent two independent Pth11-GFP expressing strains. ( C ) An RFP-trap experiment depicting the specific interaction between Pth11-mCherry and Rgs1. After probing for Pth11-mCherry with α-mCherry antibody, the same membrane was stripped and re-probed using α-Rgs1 antibody. Total protein from a cytosolic-mCherry expressing strain served as a negative control in C. ( D ) Confocal microscopy based imaging of a BiFC experiment illustrating the in-vivo interaction between Pth11-nYFP and Rgs1-cYFP in the vegetative mycelium and at 4 h post inoculation on an inductive surface (right; DIC in left panel and YFP in right panel). Asterisk indicates YFP signal and thus likely interaction at the plasma membrane. Images are single plane images captured by a confocal microscope. Scale bar, 10 µm.

Journal: PLoS Pathogens

Article Title: The Late Endosomal HOPS Complex Anchors Active G-Protein Signaling Essential for Pathogenesis in Magnaporthe oryzae

doi: 10.1371/journal.ppat.1003527

Figure Lengend Snippet: ( A ) Conidia expressing Pth11-mCherry (pseudocolored yellow) were inoculated on an inductive surface and imaged at the indicated time-points. The inset in the 2 h panel highlights a tubulo-vesicular structure containing Pth11-mCherry. The asterisk depicts the association of Pth11 with the plasma membrane. The dotted line delineates the conidial boundary. Images are maximum intensity Z-projections of five confocal stacks, measuring 0.5 µm each. Scale bar, 10 µm. ( B ) Pth11 physically interacts with Rgs1. Immunoblots from a pull down experiment depicting the specific interaction between Pth11 and Rgs1 during appressorium initiation. Pth11 physically interacted with Rgs1 during pathogenic differentiation and failed to interact with Rgs1 during vegetative growth. In each case, equal concentrations of protein from both the strains were used for the pull down experiment. Total protein from an untagged wild-type strain served as a negative control in B. S1 and S2 represent two independent Pth11-GFP expressing strains. ( C ) An RFP-trap experiment depicting the specific interaction between Pth11-mCherry and Rgs1. After probing for Pth11-mCherry with α-mCherry antibody, the same membrane was stripped and re-probed using α-Rgs1 antibody. Total protein from a cytosolic-mCherry expressing strain served as a negative control in C. ( D ) Confocal microscopy based imaging of a BiFC experiment illustrating the in-vivo interaction between Pth11-nYFP and Rgs1-cYFP in the vegetative mycelium and at 4 h post inoculation on an inductive surface (right; DIC in left panel and YFP in right panel). Asterisk indicates YFP signal and thus likely interaction at the plasma membrane. Images are single plane images captured by a confocal microscope. Scale bar, 10 µm.

Article Snippet: Equal concentration of total protein lysate was incubated with GFP/RFP-Trap agarose beads (ChromoTek, Germany) and immuno-precipitation carried out as per manufacturer's instructions.

Techniques: Expressing, Western Blot, Negative Control, Confocal Microscopy, Imaging, In Vivo, Microscopy